Purification and Partial Characterization of Maize (Zea mays L.) β-Glucosidase
نویسندگان
چکیده
منابع مشابه
Maize (Zea mays L.).
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...
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In mammals, hexokinase (HK) is strategically located at the outer membrane of mitochondria bound to the porin protein. The mitochondrial HK is a crucial modulator of apoptosis and reactive oxygen species generation. In plants, these properties related to HK are unknown. In order to better understand the physiological role of non-cytosolic hexokinase (NC-HK) in plants, we developed a purificatio...
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A glutathione S-transferase (GST) isozyme from maize (Zea mays Pioneer hybrid 3906) treated with the dichloroacetamide herbicide safener benoxacor (CGA-154281) was purified to homogeneity and partially characterized. The enzyme, assayed with metolachlor as a substrate, was purified approximately 200-fold by ammonium sulfate precipitation, anion-exchange chromatography on Mono Q resins, and affi...
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Maize (Zea mays L.) kernel pedicels, including vascular tissues, pedicel parenchyma, placento-chalazal tissue, and the surrounding pericarp, contained two forms of glutamine synthetase (EC 6.3.1.2), separable by anion exchange chromatography under mildly acidic conditions. The earlier-eluting activity (GS(p1)), but not the later-eluting activity (GS(p2)), was chromatographically distinct from t...
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The United States is the largest producer of maize in the world. Maize-soybean is the most prominent crop rotation in the Midwestern United States. Glyphosate-resistant volunteer maize is a problem weed not only in soybean, but also in continuous maize or sugarbeet rotations. Storm damage, harvesting problems, poor stalk quality, and insect damage, among other factors, can lead to kernel and ea...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1992
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.98.1.174