Purification and mechanistic characterisation of two polygalacturonases from Sclerotium rolfsii
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چکیده
منابع مشابه
Optimization of production, purification and lyophilisation of cellobiose dehydrogenase by Sclerotium rolfsii
BACKGROUND The enzyme cellobiose dehydrogenase (CDH) can be used to oxidize lactose to lactobionic acid. As Sclerotium rolfsii is known to be a good producer of CDH, the aim of this paper was to simplify its production and secondly to systematically study its purification aiming for a high yield. Two preservation methods (freezing and freeze-drying) and the influence of several protectants were...
متن کاملFirst Report of Sclerotium Rot on Cymbidium Orchids Caused by Sclerotium rolfsii in Korea
Sclerotium rot was found on Cymbidium orchids at Seosan-si, Chungcheongnam-do, Korea, in July, 2010. Symptoms occurred on low leaves, which turned yellowish, after which the entire plant wilted. Severely infected plants were blighted and eventually died. White mycelial mats and sclerotia appeared on pseudobulbs. Based on the mycological characteristics and pathogenicity, the causal fungus was i...
متن کاملOccurrence of Sclerotium Rot in Allium tuberosum Caused by Sclerotium rolfsii in Korea
In this study, we characterized sporadically occurring sclerotium rot caused by Sclerotium rolfsii in Chinese chive (Allium tuberosum Roth.) in farm fields in Sacheon, Korea. The initial symptom of the disease was water-soaked, which progressed to rotting, wilting, blighting, and eventually death. Further, mycelial mats spread over the lesions near the soil line, and sclerotia formed on the sca...
متن کاملStem Rot of Garlic (Allium sativum) Caused by Sclerotium rolfsii
Stem rot disease was found in garlic (Allium sativum L.) cultivated from 2008 to 2010 in the vegetable gardens of some farmers in Geumsan-myon, Jinju City, Gyeongnam province in Korea. The initial symptoms of the disease were typical water-soaked spots, which progressed to rotting, wilting, blighting, and eventually death. White mycelial mats had spread over the lesions near the soil line, and ...
متن کاملFormation and regeneration of protoplasts in Sclerotium rolfsii ATCC 201126.
AIMS Different cultural conditions for forming and reverting protoplasts were systematically studied to establish a rapid and efficient protocol for Sclerotium rolfsii ATCC 201126. METHODS AND RESULTS Osmotic stabilizer, lytic enzymes and mycelial age were the main factors influencing protoplast yields. An optimized protocol involving 1-h hydrolysis of 45-h-old mycelium with Trichoderma harzi...
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ژورنال
عنوان ژورنال: Enzyme and Microbial Technology
سال: 2007
ISSN: 0141-0229
DOI: 10.1016/j.enzmictec.2006.11.005