Purification and Enzymatic Properties of Glucose Isomerase fromStreptomyces griseofuscus, S-41
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چکیده
منابع مشابه
D-Xylose (D-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728. Purification and properties.
D-Xylose (D-glucose) isomerase was purified to homogeneity in yields of approx. 1 g/kg of wet cells from a strain of Arthrobacter that produces it as about 10% of total soluble protein. It is a tetramer of identical 43,114 Da subunits containing a preponderance of acidic residues and no cysteine. Partial protein sequences were determined as a step to gene cloning. It requires Mg2+, Co2+ or Mn2+...
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A glucosamine-P isomerase has been identified in Proteus mirabilis. The 113-fold purified enzyme exhibits a pH optimum of 7.5 with a secondary maximum at 8.5 and a temperature optimum at 37 degrees C. The apparent Km was 13.3 mM for fructose-6-P and 18.8 mM for L-glutamine. Molecular weight of the enzyme has been estimated as 120 000 and the protein can be dissociated in four subunits by SDS-po...
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in this thesis, at first we investigate the bounded inverse theorem on fuzzy normed linear spaces and study the set of all compact operators on these spaces. then we introduce the notions of fuzzy boundedness and investigate a new norm operators and the relationship between continuity and boundedness. and, we show that the space of all fuzzy bounded operators is complete. finally, we define...
15 صفحه اولPartial Purification & Properties of Xylose & Ribose Isomerase in Higher Plants.
The conversion of L-arabinose into the pentose moieties of plant polysaccharides proceeds wvith little rearrangement of the carbon skeleton (14,18). On the other hand D-ribose and D-xylose suffer considerable rearrangement. Experiments with specifically labeled sugars suggest that these twvo pentoses must pass through a hexose and indeed through the pentose phosphate pathway (1, 3, 8, 10, 14, 1...
متن کاملThe Purification and Properties of Streptolysin S
Considerable purification of streptolysin S has been achieved by fractionation of crude bacterial filtrates with methanol, under controlled conditions of pH, ionic strength, and temperature. The final material (P III) consisted of two electrophoretic components. The purified material was employed for kinetic studies, the results of which were confirmed with hemolysin isolated electrophoreticall...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1981
ISSN: 0002-1369
DOI: 10.1080/00021369.1981.10864571