Purification and characterization of phenoloxidase from brine shrimp Artemia sinica

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Purification and characterization of phenoloxidase from brine shrimp Artemia sinica.

Phenoloxidase from Artemia sinica (AsPO) was purified by Superdex 200 gel-filtration and Q Sepharose fast flow ion-exchange chromatography, and its properties were characterized biochemically and enzymatically by using L-dihydroxyphenylalanine (L-DOPA) as the specific substrate. Results showed that AsPO was isolated as a monomeric protein of 125.5 kDa in molecular mass. The optimal pH value and...

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Purification and properties of GTP:GTP guanylyltransferase from encysted embryos of the brine shrimp Artemia.

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The structure of Artemia sp. (brine shrimp) haemoglobins. Purification of a structural unit to homogeneity.

The extracellular haemoglobins (Mr 260 000) of the brine shrimp Artemia sp. were cleaved by limited digestion with subtilisin. Structural units of Mr 16 000, which can bind dioxygen reversibly, were isolated. Analysis of the 16 000-Mr fraction (E) reveals the presence of a limited number of structural units. A single type of structural unit, E1 (Mr 15 800; pI4.8), was purified to homogeneity an...

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Molecular phylogenetics and asexuality in the brine shrimp Artemia.

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ژورنال

عنوان ژورنال: Acta Biochimica et Biophysica Sinica

سال: 2011

ISSN: 1672-9145,1745-7270

DOI: 10.1093/abbs/gmr061