Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus
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چکیده
منابع مشابه
Purification and characterization of six fibrinolytic serine-proteases from earthworm Lumbricus rubellus.
The six lumbrokinase fractions (F1 to F6) with fibrinolytic activities were purified from earthworm Lumbricus rubellus lysates using the procedures of autolysis, ammonium sulfate fractionation, and column chromatography. The proteolytic activities on the casein substrate of the six iso-enzymes ranged from 11.3 to 167.5 unit/mg with the rank activity orders of F2 > F1 > F5 > F6 > F3 > F4. The fi...
متن کاملGrowth and reproduction of Perionyx excavatus in different organic wastes
Earthworms have been successfully used in the vermistabilization of urban, industrial and agricultural wastes in order to produce organic fertilizers and obtain protein for animal feed. Although many species could be used for these ends, given their nutritional requirements and reproductive biology, only Perionyx excavatus and Lampito mauritii are widely used. The most effective use of earthwor...
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The glycosylation of 1,3,4-oxadiazole-2-thiones has been performed with peracetylated β-pyranosyl bromide in the presence of potassium carbonate. Deprotection of acetylated thioglycosides was necessary for increasing their antibacterial effects. The structure of nucleosides were confirmed by 1H NMR, 13C NMR and HRMS. The anomeric protons of nucleosides c1–4 were assigned to the doublet, confirm...
متن کاملNovel Oxadiazole Thioglycosides as Potential Anti- Acinetobacter Agents
The glycosylation of 1,3,4-oxadiazole-2-thiones has been performed with peracetylated β-pyranosyl bromide in the presence of potassium carbonate. Deprotection of acetylated thioglycosides was necessary for increasing their antibacterial effects. The structure of nucleosides were confirmed by 1H NMR, 13C NMR and HRMS. The anomeric protons of nucleosides c1–4 were assigned to the doublet, confirm...
متن کاملCombining antithrombotic and fibrinolytic agents: can it be done?
M ore than half of ischemic stroke patients who are treated with IV recombinant tissue-type plasminogen activator (rtPA) remain disabled at 3 months. The reasons are many. The IV rtPA rate of recanalizing occluded arteries is not as high as we would like, especially for large arterial occlusions, which are opened only 30% to 40% of the time. 3–6 When rtPA does effectively lyse a thrombus, it is...
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ژورنال
عنوان ژورنال: AMB Express
سال: 2011
ISSN: 2191-0855
DOI: 10.1186/2191-0855-1-26