Purification and characterization of lysosomal H(+)-ATPase. An anion-sensitive v-type H(+)-ATPase from rat liver lysosomes.
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منابع مشابه
H-ATPase Activity from Storage Tissue of Beta vulgaris: II. H/ATP Stoichiometry of an Anion-Sensitive H-ATPase.
The H(+)/ATP stoichiometry was determined for an anion-sensitive H(+)-ATPase in membrane vesicles believed to be derived from tonoplast. Initial rates of proton influx were measured by monitoring the alkalinization of a weakly buffered medium (pH 6.13) following the addition of ATP to a suspension of membrane vesicles of Beta vulgaris L. Initial rates of ATP hydrolysis were measured in an assay...
متن کاملAnion - Sensitive , H + - Pumping ATPase in Membrane Vesicles
H+-pumping ATPases were detected in nicrosomal vesicles of oat (Avena sativa L. var Lang) roots using 114Clmethylamine distribution or quinacrine fluorescent quenching. Methylamine (MeA) accumulation into vesicles and quinacrine quench were specifically dependent on Mg,ATP. Both activities reflected formation of a proton gradient (ApH) (acid inside) as carbonyl cyanide m-chlorophenylhydrazone, ...
متن کاملDirect recruitment of H+-ATPase from lysosomes for phagosomal acidification.
The nascent phagosome progressively establishes an acidic milieu by acquiring a proton pump, the vacuolar-type ATPase (V-ATPase). However, the origin of phagosomal V-ATPase remains poorly understood. We found that phagosomes were enriched with the V-ATPase a3 subunit, which also accumulated in late endosomes and lysosomes. We modified the mouse Tcirg1 locus encoding subunit a3, to express an a3...
متن کاملTransport Properties of the Tomato Fruit Tonoplast : I. Identification and Characterization of an Anion-Sensitive H-ATPase.
An anion-sensitive H(+)-translocating ATPase was identified in membrane vesicles isolated from mature green tomato (Lycopersicon esculentum) fruit. The H(+)-ATPase was associated with a low density membrane population having a peak density of 1.11 grams per cubic centimeter, and its activity was inhibited by NO(3) (-), N,N'-dicyclohexylcarbodiimide and diethylstilbestrol but not by vanadate, az...
متن کاملAnion-sensitive, h-pumping ATPase in membrane vesicles from oat roots.
H(+)-pumping ATPases were detected in microsomal vesicles of oat (Avena sativa L. var Lang) roots using [(14)C]methylamine distribution or quinacrine fluorescent quenching. Methylamine (MeA) accumulation into vesicles and quinacrine quench were specifically dependent on Mg,ATP. Both activities reflected formation of a proton gradient (DeltapH) (acid inside) as carbonyl cyanide m-chlorophenylhyd...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)53369-x