Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
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منابع مشابه
Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
This study reports purification and characterization of lipoxygenase protein from mung bean germinating seedlings. Lipoxygenases (LOXs) are key enzymes in seed germination. The purified mung bean LOX has resolved into two peaks by chromatofocusing, one has highest LOX activity with an isoelectric point of 5.84 and the other has lowest LOX activity with an isoelectric point of 5.52. The purified...
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Two forms of Fd-NADP+ oxidoreductase (FNR) isoproteins have been purified and characterized from the first foliage leaves of 5d-old mung bean (Vigna radiata). They could be separated by either Mono Q HR 515 or ferredoxin (Fd)-Sepharose 48 affinity columns. Based on immunoblot analysis and N-terminal amino acid sequences, one form resembles the FNR purified from photosynthetic tissues of higher ...
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Serine hydroxymethyltransferase, the first enzyme in the pathway for interconversion of C(1) fragments, was purified to homogeneity for the first time from any plant source. The enzyme from 72-h mung bean (Vigna radiata L.) seedlings was isolated using Blue Sepharose CL-6B and folate-AH-Sepharose-4B affinity matrices and had the highest specific activity (1.33 micromoles of HCHO formed per minu...
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Pe]ypheno] oxidase (monophenol dLhydrexyphenylalanine' oxygen ox]doTeductase/ EC 1 14.1S t; PPO) hus been found in most higher plunts. although its ro]e has not yet becn clearly cstab]]shed. When the extracts frem var]ous organs of' mung bean seedlings were unalyzecl by :soelectric f'ocustng, several bancls of PPO ttet]vity were dctectcd. Among these isozyrnes, the one with pl S,4 showed the hi...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2016
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-016-0427-5