Purification and characterization of a new xylanase (APX-II) from the fungus Aureobasidium pullulans Y-2311-1
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منابع مشابه
Purification and characterization of a new xylanase (APX-II) from the fungus Aureobasidium pullulans Y-2311-1.
Aureobasidium pullulans Y-2311-1 produced four major xylanases (EC 3.2.1.8) with pI values of 4.0, 7.3, 7.9, and 9.4 as revealed by isoelectric focusing and zymogram analysis when grown for 4 days on 1.0% oat spelt xylan. The enzyme with a pI of 9.4 was purified by ammonium sulfate precipitation, chromatography on a DEAE-Sephadex A-50 column, and gel filtration with a Sephadex G-75 column. The ...
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Fructosyltransferase enzymes was isolated and partially purified from Aureobasidium pullulans. The enzyme being intracellular was recovered from the fungal sp. by extraction method using citrate buffer (0.1 M; pH 5.5). Enzyme was Semipurified by salt fractionation method which results increase in specific activity of the enzyme. Study regarding kinetic behavior indicates that the enzyme exhibit...
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The extracellular polygalacturonases produced by Aureobasidium pullulans isolated from waters of the Danube river were partially purified and characterized. The pH optima of polygalacturonases produced in the first phases of cultivation (48 h) and after 10 d as well as their optima of temperature, thermal stabilities, molecular masses, isoelectric points, action pattern and ability to cleave po...
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UNLABELLED Objective(s) : Although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed. MATERIALS AND METHODS Single xylanase producer yeast was isolated from plant residues based on formation of transparent halo zones on xylan agar plates. The isolate showed much greate...
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A color-variant strain of Aureobasidium pullulans (NRRL Y-12974) produced alpha-L-arabinofuranosidase (alpha-L-AFase) when grown in liquid culture on oat spelt xylan. An extracellular alpha-L-AFase was purified 215-fold to homogeneity from the culture supernatant by ammonium sulfate treatment, DEAE Bio-Gel A agarose column chromatography, gel filtration on a Bio-Gel A-0.5m column, arabinan-Seph...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1993
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.59.10.3212-3218.1993