Purification and characterization of a chitinase from Trichoderma viride.
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چکیده
منابع مشابه
Purification and characterization of chitinase from Streptomyces sp. M-20.
Chitinase (EC 3.2.1.14) was isolated from the culture filtrate of Streptomyces sp. M-20 and purified by ammonium sulfate precipitation, DEAE-cellulose ion-exchange chromatography, and Sephadex G-100 gel filtration. No exochitinase activity was found in the culture filtrate. The molecular mass of the purified chitinase was 20 kDa, estimated by a sodium dodecyl sulfate-polyacrylamide gel electrop...
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Plants exhibit an altered pattern of protein synthesis in response to pathogen invasion and abiotic stress. One of these ;pathogenesis-related' proteins has been identified as chitinase, which is capable of inhibiting fungal growth in vitro. This observation has led to the suggestion that the in vivo role of chitinases is to protect plants against fungal invasion. Here, we report the purificati...
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Four Chitin degrading bacterial strains vibrio aestuarianus, Flavobacterium, Shewenella and Exiguobacterium were isolated from crustacean shells. The strains were confirmed by Biochemical analysis, FAME-GC analysis and 16s rDNA sequencing. The chitinase was purified by a two step chromatographic method and characterized. The enzyme was purified to homogeneity by 6.75 fold with 46% recovery afte...
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Investigations on the development of a biocontrol agent against the alarming stored food pest Corcyra cephalonica has witnessed noteworthy growth in current years from mainly physiological studies, to tackling fundamental queries concerning the underlying mode of action. Chitinase from Trichoderma viride, which has inauspicious effects on feeding behavior, development and biochemical parameters...
متن کاملNucleotide sequence of the cellobiohydrolase gene from Trichoderma viride.
The filamentous fungus, Trichoderma viride, secretes large amounts of several cellulolytic enzymes (1). We report here the nucleotide sequence of the gene coding for exo-cellobiohydrolase (CBH), which accounts for more than 65% of the total cellulase content. This gene was isolated from a T. viride genomic library using a 30mer oligonucleotide based on the sequence of the reported CBHI gene of ...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 2001
ISSN: 0022-1260,1349-8037
DOI: 10.2323/jgam.47.53