Purification and characterisation of thermostable α-amylases from microbial sources

a-Amylases from Microbial Sources – An Overview on Recent Developments

This review covers the progress made in research on microbial a-amylase, a highly demanded industrial enzyme in various sectors such as food, pharmaceuticals, textiles, detergents, etc. Amylases are of ubiquitous occurrence and hold the maximum market share of enzyme sales. The article surveys the a-amylase family and the major characteristics, microbial sources, production aspects, downstream ...

Purification and Characterization of a Novel Thermostable and Acid Stable α-Amylase from Bacillus Sp. Iranian S1

This study reports the purification and biochemical characterization of thermostable and acidic-pH-stable α-amylase from Bacillus sp. Iranian S1 isolated from the desert soil (Gandom-e-Beryan in Lut desert, Iran). Amylase production was found to be growth associated. Maximum enzyme production was in exponential phase with activity 2.93 U ml-1 at 50°C and pH 5. The enzyme was purified by isoprop...

Alpha-amylase inhibitor of amadumbe (Colocasia esculenta): Isolation, purification and selectivity toward - amylases from various sources

Two proteins (A-1 and B-2) with -amylase inhibitor activity were extracted and partially purified from Colocasia esculenta tubers through 80% ammonium sulphate fractionation, ion-exchange chromatography on DEAE -Sephacel and gel-chromatography on Sephadex G-100. The molecular weight of A-1 and B-2 were estimated to be about 17000 and 19000 daltons, respectively. The inhibitors inactivated -am...

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

Purification and Characterization of Thermostable and Detergent-Stable α-Amylase from Anoxybacillus sp. AH1.

A thermostable and detergent-stable α-amylase from a newly isolated Anoxybacillus sp. AH1 was purified and characterized. Maximum enzyme production (1874.8 U/mL) was obtained at 24 h of incubation. The amylase was purified by using Sephadex G-75 gel filtration, after which an 18-fold increase in specific activity and a yield of 9% were achieved. The molecular mass of the purified enzyme was est...