Purification and caracterization of DNA polymerase alpha from plasmodia of Physarum polycephalum
نویسندگان
چکیده
منابع مشابه
Growth of large plasmodia of the myxomycete Physarum polycephalum.
A method has been developed for growing Physarum polycephalum plasmodia that are 8 to 10 times larger than those obtained in the petri dish cultures used by Nygaard, Guttes, and Rusch. In the large-scale procedure, plasmodia were grown in metal trays on a membrane supported by filter paper on stainless-steel screen. Plasmodia were started from a ring of inoculum to allow inward and outward migr...
متن کاملPost-fusion somatic incompatibility in plasmodia of Physarum polycephalum.
High-resolution autoradiography has been used to establish that during the incompatibility reaction that follows fusion between plasmodia of a 'killer' and a 'sensitive' strain of the myxomycete Physarum polycephalum, the nuclei of the sensitive strain are selectively damaged, enclosed in vacuoles and eliminated from the cytoplasm. This damage is visible as increased chromatin condensation and ...
متن کاملPost-fusion Incompatibility in Plasmodia of Physarum Polycephalum
In the myxomycete Physarum polycephalum the somatic incompatibility between plasmodia of a killer strain and a sensitive strain is expressed as a lethal reaction (LR) in the sensitive strain within 6 h after fusion of both plasmodia. The lethal reaction was not caused by any of the extra-cellular compounds produced by the myxomycete nor was the result of intra-cellular compounds of the killer s...
متن کاملPurification and properties of two RNA polymerases from Physarum polycephalum.
Two RNA polymerases have been purified from the slime mold Physarum polycephalum, one sensitive and one resistant to alpha-amanitin. Both enzymes are more active with denatured DNA than native DNA as a template and prefer Mn(++) rather than Mg(++) as a divalent cation. The alpha-amanitin-sensitive enzyme shows maximum activity at 0.15 M KCl, whereas the resistant enzyme is most active at very l...
متن کاملPurification and properties of ornithine decarboxylase from Physarum polycephalum.
Ornithine decarboxylase (EC 4.1.1.17) has been purified 3,500-fold from the plasmodia of Physarum polycephalum. The purified material exhibited a Km for ornithine of 0.6 mM and Vmax of 20 mumol of CO2 formed per min/mg at 30 degrees C (62 mumol/min/mg at 37 degrees C). It migrated as a single protein and activity species on high pressure liquid chromatography (TSK-3000) in 0.15 M NaCl (Mr = 80,...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1988
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1988.tb14269.x