PTEN is required for the migration and invasion of Ras‐transformed MDCK cells

The balance between phosphoinositides distributed at specific sites in the plasma membrane causes polarized actin polymerization. Oncogenic transformations affect this by regulating phosphoinositide 3-kinase (PI3K) and phosphatase tensin homolog deleted on chromosome 10 (PTEN), causing metastatic behavior cancer cells. Here, we show that PTEN tumor suppressor gene is required for epithelial cell invasion. Loss of Ras-transformed MDCK cells suppressed their migratory phenotype collagen gel invasion through Matrigel. Rescue experiments showed a requirement C2 domain-mediated recruitment PTEN, which typically observed rear side invading These findings support role suppression unwanted leading edges necessary efficient migration