Pseudodesulfovibrio alkaliphilus, sp. nov., an alkaliphilic sulfate-reducing bacterium isolated from a terrestrial mud volcano

The diversity of anaerobic microorganisms in terrestrial mud volcanoes is largely unexplored. Here we report the isolation a novel sulfate-reducing alkaliphilic bacterium (strain F-1T) from volcano located at Taman peninsula, Russia. Cells strain F-1T were Gram-negative motile vibrios with single polar flagellum; 2.0–4.0 µm length and 0.5 diameter. temperature range for growth was 6–37 °C, an optimum 24 °C. pH 7.0–10.5, 9.5. Strain utilized lactate, pyruvate, molecular hydrogen as electron donors sulfate, sulfite, thiosulfate, elemental sulfur, fumarate or arsenate acceptors. In presence end products lactate oxidation acetate, H2S CO2. Lactate pyruvate could also be fermented. major product fermentation acetate. main cellular fatty acids anteiso-C15:0, C16:0, C18:0, iso-C17:1ω8. Phylogenetic analysis revealed that most closely related to Pseudodesulfovibrio aespoeensis (98.05% similarity). total size genome isolate 3.23 Mb genomic DNA G + C content 61.93 mol%. contained all genes essential dissimilatory sulfate reduction. We propose assign genus Pseudodesulfovibrio, new species, alkaliphilus sp. nov. type (= KCTC 15918T = VKM B-3405T).