Proximity Labeling at Non-Centrosomal Microtubule-Organizing Centers Reveals VAB-10B and WDR-62 as Distinct Microtubule Regulators
نویسندگان
چکیده
منابع مشابه
Distribution of Microtubule Organizing Centers
This study was designed to investigate the relationship between the position of the microtubule organizing center (MTOC) and the direction of migration of a sheet of endothelial cells (EC) . Using immunofluorescence and phase microscopy the MTOC's of migrating EC were visualized as the cells moved into an in vitro experimental wound produced by mechanical denudation of part of a confluent monol...
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The centrosome consists of a pair of centrioles surrounded by pericentriolar material. During the formation of the mitotic spindle, multi-protein complexes in the pericentriolar material are involved in the nucleation and anchorage of microtubules. In postmitotic cells of many tissues, proteins of the pericentriolar material lose their association with the centrosome and redistribute to various...
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Differentiation of columnar epithelial cells involves a dramatic reorganization of the microtubules (MTs) and centrosomal components into an apico-basal array no longer anchored at the centrosome. Instead, the minus-ends of the MTs become anchored at apical non-centrosomal microtubule organizing centres (n-MTOCs). Formation of n-MTOCs is critical as they determine the spatial organization of MT...
متن کاملMicrotubule Nucleation at Non-Spindle Pole Body Microtubule-Organizing Centers Requires Fission Yeast Centrosomin-Related Protein mod20p
BACKGROUND Many types of differentiated eukaryotic cells display microtubule distributions consistent with nucleation from noncentrosomal intracellular microtubule organizing centers (MTOCs), although such structures remain poorly characterized. In fission yeast, two types of MTOCs exist in addition to the spindle pole body, the yeast centrosome equivalent. These are the equatorial MTOC, which ...
متن کاملMicrotubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein.
The novel concept of a centrosomal anchoring complex, which is distinct from the gamma-tubulin nucleating complex, has previously been proposed following studies on cochlear epithelial cells. In this investigation we present evidence from two different cell systems which suggests that the centrosomal protein ninein is a strong candidate for the proposed anchoring complex. Ninein has recently be...
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ژورنال
عنوان ژورنال: SSRN Electronic Journal
سال: 2020
ISSN: 1556-5068
DOI: 10.2139/ssrn.3699793