Prototyping protein expression constructs with PCR, cell-free expression and fluorescence detection size exclusion chromatography

Organic matter from biofilter nitrification by high performance size exclusion chromatography and fluorescence excitation-emission matrix

A combination of high performance size exclusion chromatography with organic carbon detector and ultraviolet detector coupled with peak-fitting technique and fluorescence excitation-emission matrix spectrometry applied fluorescence regional integration method was conducted to determine the characteristics of organic matter during nitrification. The batch scale of bionet nitrification without or...

A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening.

Optimization of membrane protein stability under different solution conditions is essential for obtaining crystals that diffract to high resolution. Traditional methods that evaluate protein stability require large amounts of material and are, therefore, ill suited for medium- to high-throughput screening of membrane proteins. Here we present a rapid and efficient fluorescence-detection size-ex...

Is Salt-Free Size-Exclusion Chromatography Ready?

Size-Exclusion Chromatography of Polysaccharides with Pulsed Amperometric Detection (PAD)

INTRODUCTION Size-exclusion chromatography is used in carbohydrate research to determine molecular weight distributions of polysaccharides. A variety of detection methods have been used, among them viscometry, lowangle laser light scattering (LALLS), refractive index, ultraviolet/visible absorbance, fluorescence, and precision differential refractometry coupled with ultraviolet detection.This a...

Size-Exclusion Chromatography of Polysaccharides with Pulsed Amperometric Detection (PAD)

INTRODUCTION Size-exclusion chromatography is used in carbohydrate research to determine molecular weight distributions of polysaccharides. A variety of detection methods have been used, among them viscometry, lowangle laser light scattering (LALLS), refractive index, ultraviolet/visible absorbance, fluorescence, and precision differential refractometry coupled with ultraviolet detection.This a...