Protochlorophyll(IDE) holochrome subunits from a mutant defective in the regulation of protochlorophyll(IDE) synthesis
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چکیده
منابع مشابه
Purification of protochlorophyllide holochrome.
Phototransformable protochlorophyllide holochrome was prepared from etiolated bean leaves. The detergent Triton X-100 in the presence of glycerol and tricine-KOH buffer (pH 8) enhanced the extractability, specific activity, and phototransformability of the holochrome. Purification was achieved by polyethylene glycol-6000 precipitation and hydroxyl-apatite, DEAE-cellulose, and agarose chromatogr...
متن کاملEnzymic nature of the protein moiety of protochlorophyllide holochrome.
The enzymic nature of the protein moiety of protochlorophyll(ide) holochrome was studied by following the fate of the [(14)C]protochlorophyll(ide) formed when dark-grown barley (Hordeum vulgare) or bean (Phaseolus vulgaris) leaves are incubated in the dark with 3 mm 4-delta-[(14)C]aminolevulinic acid. It was found that: [List: see text]Since turnover of protochlorophyll(ide) was not observed, t...
متن کاملEnergy Transfer from NADPH to Protochlorophyllide in Isolated Protochlorophyllide Holochrome as Determined by UV-Fluorescence Excitation Spectroscopy at 77 K
The low -tem perature fluorescence excitation analysis o f different pro tochlorophyllide (PChlide) form s has been extended to the UV part o f the spectrum . A new band at abou t 360 nm was detected in excitation spectra o f active PChlide forms bound to isolated p ro to chlorophyllide holochrom e. This band is very similar to the absorbance o f N A D P H in this region and its intensity dep...
متن کاملImmunological distinction between fraction I protein and protochlorophyllide holochrome.
Ribulose 1,5-diphosphate carboxylase (RuDP carboxylase) has been shown to be the major component of the Fraction I protein of leaves (5, 6). Protochlorophyllide associated with a protein, i.e. protochlorophyllide holochrome (PCH), has been isolated from etiolated bean leaves (1, 9,10); upon illumination in zirvo or in vitro it is transformed into chlorophyllide holochrome. Several investigators...
متن کاملAnalysis of the subunit structure of protochlorophyllide holochrome by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
The subunit structures of protochlorophyllide holochrome (PCH) and chlorophyllide holochrome (CH) were studied by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. PCH from leaves of dark-grown (Phaseolus vulgaris var. red kidney) is a polymeric pigment-protein complex of approximately 600,000 daltons. It is composed of 12 to 14 polypeptides of 45,000 daltons, when examined prior...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1973
ISSN: 0014-5793
DOI: 10.1016/0014-5793(73)80517-4