Proteomic Analysis on Sequential Samples of Cystic Fluid Obtained from Human Brain Tumors

Cystic formation in human primary brain tumors is a relatively rare event whose incidence varies widely according to the histotype of tumor. Composition cystic fluid has mostly been characterized samples collected at time tumor resection and no indications evolution content are available. We proteome using bottom-up proteomic approach on sequential obtained from secretory meningioma (SM), schwannoma (CS) high-grade glioma (CG). identified 1008 different proteins; 74 these proteins were found least once all tumors. The most abundant common studied derived plasma, with exception prostaglandin D2 synthase, which marker cerebrospinal origin. Overall, protein composition times same remained stable. After identification differentially expressed (DEPs) protein–protein interaction network analysis, we presence tumor-specific pathways that may help characterize tumor–host interactions. Our results suggest plasma leaking local blood–brain barrier disruption important contributors cyst formation, but (CSF) itself also contribute and, some cases, as immunoglobulin G, shows variations cannot be simply explained by differences vessel permeability or blood contamination.