Proteolytic activity of partly purified ribonuclease inhibitor from rat liver
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چکیده
منابع مشابه
Ribonuclease inhibitor and latent ribonuclease in rat liver during feeding of 2-acetamidofluorene.
the relationships between RNase activity and cancer, and a review concerning these relationships has been published (14). An understanding of the role of RNase in growth processes has been complicated by dernonstra tions that there is a multiplicity of RNases (14) and of intracellular inhibitors for RNase (9, 11) in animal tissues. Furthermore, the recent discovery that some samples of RNA cont...
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The ribonuclease and phosphodiesterase activities of rat liver plasma membranes, purified from the crude nuclear fraction by centrifugation in an A-XII zonal rotor and flotation, were examined and compared. The plasma membrane is responsible for between 65 and 90% of the phosphodiesterase activity of the cell and between 25 and 30% of the particulate ribonuclease activity measured at pH8.7 in t...
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A qualitative and quantitative characterization of the proteolytic activity of a number of rat liver cell fractions was undertaken using lY51-labeled hemoglobin as substrate. The results indicated that proteolytic activity in rat liver was of two major types. The first was characteristic of activity accounted for by the endopeptidase cathepsin I). It was sensitive to the inhibitor pepstatin, an...
متن کاملProteolytic Contaminants of Crystalline Ribonuclease
1. The ability of crystalline ribonuclease to hydrolyze proteins is due to impurities present in the preparations and not to an intrinsic property of the ribonuclease molecule. The amounts of these impurities vary enormously with different preparations. 2. The dangers inherent in the use of crystalline enzymes as specific tools, without assaying them for all possible interfering impurities, are...
متن کاملV. Ribonuclease Activity in Ribosomes and Polysomes Prepared from Rat Liver and Hepatomas
The RNase activity and properties of ribosome and polysomc preparations from normal rat liver and some hepatomas havc been examined. Polysomc and ribosome preparations from the Novikoff, McCoy MDAB, and Dunning hcpatomas had considerably higher specific RNase activity than corresponding preparations from normal rat livcr, Novikoff ascites, or Morris 5123 hcpatomas. The optimum pH of the RNasc w...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
سال: 1971
ISSN: 0005-2787
DOI: 10.1016/0005-2787(71)90807-0