Proteolysis of rabbit immunoglobulin M by papain
نویسندگان
چکیده
منابع مشابه
Characterization of a large fragment produced by proteolysis of human immunoglobulin M with papain.
The controlled digestion of human immunoglobulin M (IgM) (Patient Dau.) with papain in the presence of 0.002 M mercaptoethylamine resulted in the release of a basic subunit-like fragment which we designated IgM,. It resembled the subunit (IgM,) produced by mild reduction of IgM in the following ways. The molecular weight (186,000) and sedimentation coefficient (6.2 to 6.5 S) of IgM, were simila...
متن کاملStepwise cleavage of rabbit immunoglobulin G by papain and isolation of four types of biologically active Fc fragments.
Four types of Fc fragments of different sizes were isolated by papain treatment of rabbit immunoglobulin G under various conditions and by subsequent chromatographic procedures. 1. Brief digestion at neutral pH without reduction produced a molecule in which the Fab and Fc fragments were still linked by a pair of labile disulphide bridges, and the Fc fragment released by cleaving these bonds, ca...
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Some biochemical aspects of the collapse of the rabbit ears produced by the intravenous injection of papain have been studied. A marked depletion of chondromucoprotein (M.C.S.) and a reduction of the S(35) content of cartilage matrix were found to coincide with the gross and histologic changes in the cartilage. At the same time there was a marked increase in the amount of S(35) in the serum and...
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As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-D.35M NaCI ...
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The present studies were designed to determine the effect of papain upon IgM and IgD on the surface of murine splenocytes. By using radioiodinated cells, it was shown that IgD is more susceptible to cleavage by papain than IgM. The difference in susceptibility to papain of the two isotypes was not observed when cell lysates were employed, suggesting that attachment to the plasma membrane affect...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1974
ISSN: 0264-6021
DOI: 10.1042/bj1430491