Protein renaturation by the liquid organic salt ethylammonium nitrate
نویسندگان
چکیده
منابع مشابه
Properties of Hydrogen Bonds in the Protic Ionic Liquid Ethylammonium Nitrate DFT versus DFTB Molecular Dynamics
Comparative molecular dynamics simulations of a hexamer cluster of the protic ionic liquid ethylammonium nitrate are performed using density functional theory (DFT) and density functional-based tight binding (DFTB) methods. The focus is on assessing the performance of the DFTB approach to describe the dynamics and infrared spectroscopic signatures of hydrogen bonding between the ions. Average g...
متن کاملStructure of nonionic surfactant micelles in the ionic liquid ethylammonium nitrate.
The structure of micelles formed by nonionic polyoxyethylene alkyl ether nonionic surfactants, C n E m , in the room-temperature ionic liquid, ethylammonium nitrate (EAN), has been determined by small-angle neutron scattering (SANS) as a function of alkyl and ethoxy chain length, concentration, and temperature. Micelles are found to form for all alkyl chains from dodecyl through to octadecyl. D...
متن کاملAcidity and basicity of aqueous mixtures of a protic ionic liquid, ethylammonium nitrate.
Ethylammonium nitrate (EAN) is composed of C(2)H(5)NH(3)(+) and NO(3)(-) ions, which behave as an acid and a base, respectively. The ionic liquid thus involves small amounts of C(2)H(5)NH(2) and HNO(3) molecules owing to proton transfer from C(2)H(5)NH(3)(+) to NO(3)(-). The equilibrium constant K(s) (= [C(2)H(5)NH(2)][HNO(3)]), which corresponds to the autoprotolysis constant of water, was obt...
متن کاملFabrication of different morphologies of ZnO superstructures in presence of synthesized ethylammonium nitrate (EAN) ionic liquid: synthesis, characterization and analysis.
ZnO particles were synthesized by hydrothermal route at 95 °C, with different ethylammonium nitrate (EAN) : water volume ratio (0.01 to 1) in reaction media. Morphology of ZnO particles changed from initial cylindrical to intermediate spindle and finally to spherical with increasing concentration of EAN in reaction media whereas pH of aliquots remained within range of 7-7.5. Aggregates of EAN b...
متن کاملThe solubility of hen lysozyme in ethylammonium nitrate/H2O mixtures and a novel approach to protein crystallization.
We report on the solubility of hen lysozyme (HEWL) in aqueous ethylammonium nitrate (EAN) as a function of water content. We find the solubility behavior to be complex, exhibiting both a maximum (400 mg/mL) at very high EAN content) and a minimum at intermediate EAN content. We exploit this solubility profile in a novel approach to generating crystals of hydrophilic proteins, based on rehydrati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protein Science
سال: 2000
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.9.10.2001