Protein Hydrolysates from Flaxseed Oil Cake as a Media Supplement in CHO Cell Culture

Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture

Silk protein sericins, in the recent years, find application in cosmetics and pharmaceuticals and as biomaterials. We investigate the potential of sericin, extracted from both mulberry Bombyx mori and different non-mulberry sources, namely, tropical tasar, Antheraea mylitta; muga, Antheraea assama; and eri, Samia ricini, as growth supplement in serum-free culture medium. Sericin supplemented me...

Novel Cell-Ess ® supplement used as a feed or as an initial boost to CHO serum free media results in a significant increase in protein yield and production

Many metrics, including metabolic profiles, have been used to analyze cell health and optimize productivity. In this study, we investigated the ability of a lipid supplement to increase protein yield. At a concentration of 1% (v/v) the lipid supplement caused a significant increase in protein titer (1118 ± 65.4 ng 10(5) cells(- 1) days(- 1)) when compared to cultures grown in the absence of sup...

Urea-treated neem (Azadirachta indica A. juss) seed kernel cake as a protein supplement for lambs

The performance of 12 male and 12 female Mandya Merino lambs weighing between 5.4 and 12.4 kg was investigated by feeding neem seed kernel cake (NSKC) after treatment with 2.5% urea. Lambs of each sex were allotted randomly to concentrate mixture containing either 30% groundnut cake (GNC) or 33% urea-treated neem seed kernel cake (UTNSKC). Over a period of 182 days the concentrate mixtures fed,...

Protein Hydrolysates as Transfusion Material

Rice bran extract (RBE) as supplement for cell culture

Introduction In mammalian cell culture, fetal bovine serum (FBS) or proteins obtained from mammals are usually supplemented to culture media. Since the use of animal-derived components may cause an infection of virus and other pathogens, alternative supplement derived from nonmammals is eagerly required in cell culture for producing biotherapeutics and for cell therapy [1]. As an alternative su...