Protein Engineering of Mung Bean (Vigna radiata (L.) Wilczek) 8Sα Globulin with Lactostatin
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چکیده
منابع مشابه
Mung Bean (Vigna radiata) Seedlings'
Two forms of Fd-NADP+ oxidoreductase (FNR) isoproteins have been purified and characterized from the first foliage leaves of 5d-old mung bean (Vigna radiata). They could be separated by either Mono Q HR 515 or ferredoxin (Fd)-Sepharose 48 affinity columns. Based on immunoblot analysis and N-terminal amino acid sequences, one form resembles the FNR purified from photosynthetic tissues of higher ...
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Cold-induced changes in vivo in the cytoplasmic pH of suspension-cultured cells of mung bean (Vigna radiata [L.] Wilczek) were investigated by fluorescence-ratio imaging cryomicroscopy with special reference to the variations in the chilling sensitivity of cells during the growth cycle. Because of the preferential localization of the fluorophore in the cytoplasm under specified conditions and t...
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A pot culture experiment was conducted in mung bean (Vigna radiata (L.) Wilczek.) genotype PUSA Baisakhi to test the effect of sodium arsenate and thiourea (TU). Arsenic at 25 μM caused significant inhibition of growth as indicated by reduced shoot and root dry weight and reduction in photosynthetic capacity. Significant decline in ascorbate peroxidase, dehydroascorbate reductase and glutathion...
متن کاملBiosynthesis of Phosphatidylglycerol in Isolated Mitochondria of Etiolated Mung Bean (Vigna radiata L.) Seedlings.
Phosphatidylglycerophosphate synthase (sn-glycerol-3-phosphate:CDP-diacylglycerol phosphatidyltransferase) and phosphatidylglycerophosphate phosphatase were characterized in mung bean (Vigna radiata L.) mitochondria. The synthase has a rather broad pH optimum between 7 and 9, whereas the phosphatase has one of about 7. Both enzymic activities are stimulated by Triton X-100 and require divalent ...
متن کاملPurification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
This study reports purification and characterization of lipoxygenase protein from mung bean germinating seedlings. Lipoxygenases (LOXs) are key enzymes in seed germination. The purified mung bean LOX has resolved into two peaks by chromatofocusing, one has highest LOX activity with an isoelectric point of 5.84 and the other has lowest LOX activity with an isoelectric point of 5.52. The purified...
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ژورنال
عنوان ژورنال: Applied Sciences
سال: 2020
ISSN: 2076-3417
DOI: 10.3390/app10248787