Protein composition of Rhodopseudomonas sphaeroides outer membrane
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چکیده
منابع مشابه
Solubilization, isolation, and immunochemical characterization of the major outer membrane protein from Rhodopseudomonas sphaeroides.
Solubilization of the major outer membrane protein of Rhodopseudomonas sphaeroides, and subsequent isolation, has been achieved by both non-detergent- and detergent-based methods. The protein was differentially solubilized from other outer membrane proteins in 5 M guanidine thiocyanate which was exchanged by dialysis for 7 M urea. The urea-soluble protein was purified to homogeneity by a combin...
متن کاملHeat modifiability and detergent solubility of outer membrane proteins of Rhodopseudomonas sphaeroides.
The outer membrane fraction from Rhodopseudomonas sphaeroides was isolated by isopycnic density centrifugation. The purity of this fraction was assayed by several methods. When the outer membrane fraction obtained after French press lysis of cells was compared with the outer membrane fragments released during spheroplast formation, the polypeptide profiles were identical. Detergent solubilizati...
متن کاملEffects of incident light levels on photosynthetic membrane polypeptide composition and assembly in Rhodopseudomonas sphaeroides.
Cells of Rhodopseudomonas sphaeroides were grown anaerobically with incident light levels ranging between 4,500 and 400 footcandles (ca. 48,420 and 4,304 lux). Cells grown with the higher light levels had lower contents of total bacteriochlorophyll and incorporated L-[U-14C]leucine into membrane protein at higher rates than cells grown with lower light levels. The former cells also contained re...
متن کاملIntracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Polypeptide insertion into growing membrane.
A nonspecific density labeling technique has been employed to monitor the synthesis of intracytoplasmic membrane in synchronously dividing populations of Rhodopseudomonas sphaeroides. The intracytoplasmic membranes of cells synchronized in D2O-based medium were found to undergo discontinuous decreases in specific density during synchronous cell growth following transfer to H2O-based medium. The...
متن کاملDegradation of Bacteriochlorophyll a in Rhodopseudomonas sphaeroides
J. Ullrich Physikalisches Institut der Universität, Pfaffenwaldring 57, D-7000 Stuttgart Z. Naturforsch. 40c, 685 — 692 (1985); received May 24, 1985 Rhodopseudomonas sphaeroides, Bacteriochlorophyll a, Bacteriopheophytin a, Bacteriopheophorbide a, Pigment aggregation A series of pigments of the bacteriopheophytin a spectral type have been isolated from ageing cultures of Rhodopseudomonas sphae...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1980
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.143.1.265-273.1980