Protective Effect of Ectoin on UVA/H2O2-Induced Oxidative Damage in Human Skin Fibroblast Cells

Ectoin is an amino acid derivative that can create a balance between the osmotic pressure of cells and protect enzymes, DNA proteins, nucleic acids under extreme conditions. has also been reported to slow skin aging. However, there are few reports on protective effect oxidative damage, especially regulation PI3K/AKT-pathway-related genes at mRNA level. UVA-induced stress injury H2O2-induced two common models. Skin fibroblasts produce large number ROS following excessive UV radiation or stimulation by H2O2, which further inhibits cell proliferation causes apoptosis. In this study, UVA- oxidation models human were established separately investigate Ectoin. Further studies mechanisms involved, for example, expression levels associated with PI3K/AKT signaling pathway antioxidant enzymes in cells, determined. We found upregulated pathway, including COL1A1, COL1A2, FN1, IGF2, NR4A1, PIK3R1, but decreased intracellular malondialdehyde (MDA), while increasing activities superoxide dismutase (SOD) glutathione peroxidase (GSH-Px). conclusion, our results indicate exerts properties PIK3R1 upregulating antioxidative enzyme levels.