Protamine immobilization and heparin adsorption on the protamine-bound cellulose fiber membrane
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چکیده
منابع مشابه
Protamine immobilization and heparin adsorption on the protamine-bound cellulose fiber membrane.
Immobilization of protamine to the inner lumen of cellulose hollow fibers has been shown useful in preventing both heparin- and protamine-induced complications during an extracorporeal blood circulation procedure. The current study examined the effects of variables on the immobilization of protamine to cyanogen bromide (CNBr)-activated cellulose hollow fibers. The degree of protamine immobiliza...
متن کاملPoly-L-lysine amplification of protamine immobilization and heparin adsorption.
We previously reported the development of a cellulose fiber based blood filter device containing immobilized protamine (termed protamine filter) that could be used to control both heparin- and protamine-induced complications during extracorporeal therapy. To achieve enhanced heparin adsorption on the fibers, we examined the possibility of utilizing the poly-L-lysine based amplification method t...
متن کاملImproved protamine-sensitive membrane electrode for monitoring heparin concentrations in whole blood via protamine titration.
An improved protamine-sensitive electrode based on a polymeric membrane doped with the charged ion exchanger dinonylnaphthalenesulfonate (DNNS) is used for monitoring heparin concentrations in whole blood. The electrode exhibits significant nonequilibrium potentiometric response to polycationic protamine over the concentration range of 0.5-20 mg/L in undiluted whole-blood samples. The sensor ca...
متن کاملEffect of protamine sulfate on the aca heparin assay.
Fig. 1 . Effect of increasing amounts of protamine sulfate on the ace measurement of hepann in whole blood (1 USP unlt/mL) The protam,ne/hepann ratio is based on the assumption that 0.01 mg of protamine neutralizes 1 unIt of hepaiin. The measured hepaiin concentration is shown as a percent of anti-factor Xa activity relative to baseline (no added protamine) determined in each experiment. The re...
متن کاملHigh incidence of antibodies to protamine and protamine/heparin complexes in patients undergoing cardiopulmonary bypass.
Protamine is routinely used to reverse heparin anticoagulation during cardiopulmonary bypass (CPB). Heparin interacts with protamine to form ultralarge complexes that are immunogenic in mice. We hypothesized that patients exposed to protamine and heparin during CPB will develop antibodies (Abs) to protamine/heparin (PRT/H) complexes that are capable of platelet activation. Specimens from a rece...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 1992
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.260390412