Propidium iodide quenches the fluorescence of TdT‐incorporated FITC‐labeled dUTP in apoptotic cells
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منابع مشابه
Effect of adriamycin and analogs on the nuclear fluorescence of propidium iodide-stained cells.
Adriamycin (ADR) and N-trifluoroacetyladriamycin-14-valerate, respectively, inhibit and enhance the nuclear fluorescence of cells stained with propidium iodide for DNA per cell estimation by flow cytometry. In cells incubated with ADR, the reduction in fluorescence is gradually manifested due to the slow intracellular drug transport. In contrast the effect of N-trifluoroacetyladriamycin-14-vale...
متن کاملA pitfall of propidium iodide staining in fluorescence-activated cell sorting cell cycle analysis?
We read with great interest the recent article by Fischer et al. (1) on uracil-DNA glycosylase and its expression modulated by fluorodeoxyuridine (FdUrd). As a particular observation, the authors provide evidence that FdUrd-induced uracil-DNA glycosylase degradation occurred in late G1-early S transition instead of late S-G2-M phase as was observed previously in normally cycling cells (2). This...
متن کاملPropidium iodide as an indicator of Giardia cyst viability.
The use of propidium iodide, whose uptake indicates cell death or damage, was investigated to assess the viability of heat-inactivated and chemically inactivated Giardia muris cysts. This was done by comparing propidium iodide staining with excystation. We first determined that propidium iodide could be used with an immunofluorescence detection procedure by showing that the percentages of Giard...
متن کاملProgressive incorporation of propidium iodide in cultured mouse neurons correlates with declining electrophysiological status: a fluorescence scale of membrane integrity.
We describe a visual assay of neuronal electrophysiologic status for use with cultured neurons, based on the exclusion of propidium iodide (PI) by intact cellular membranes. We use this fluorescent dye, which binds to nucleic acids, at concentrations suitable for long-term exposure to neurons without toxicity. We correlate the progressive loss of resting membrane potential and the progressive i...
متن کاملAnthocyanin inhibits propidium iodide DNA fluorescence in Euphorbia pulcherrima: implications for genome size variation and flow cytometry.
BACKGROUND Measuring genome size by flow cytometry assumes direct proportionality between nuclear DNA staining and DNA amount. By 1997 it was recognized that secondary metabolites may affect DNA staining, thereby causing inaccuracy. Here experiments are reported with poinsettia (Euphorbia pulcherrima) with green leaves and red bracts rich in phenolics. METHODS DNA content was estimated as flu...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1998
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(19981201)33:4<428::aid-cyto6>3.3.co;2-w