Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells

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Properties of connexin 46 hemichannels in dissociated lens fiber cells.

PURPOSE To characterize the properties of connexin 46 hemichannels in differentiating fiber cells isolated from mouse lenses. METHODS Differentiating fiber cells were isolated from mouse lenses using collagenase. Cellular localization of connexin 50 (Cx50) and connexin 46 (Cx46) was assessed by immunofluorescence. Membrane currents were recorded using whole cell patch clamping. Dye uptake was...

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Connexin hemichannels and cell-cell channels: comparison of properties.

Connexin46 (Cx46) forms functional hemichannels in the absence of contact by an apposed hemichannel and we have used these hemichannels to study gating and permeation at the single channel level with high time resolution. Using both cell-attached and -excised patch configurations, we find that single Cx46 hemichannels exhibit some properties expected of half of a gap junction channel, as well a...

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ژورنال

عنوان ژورنال: Investigative Opthalmology & Visual Science

سال: 2011

ISSN: 1552-5783

DOI: 10.1167/iovs.10-6200