Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads

The safety of the cells used for Advanced Therapy Medicinal Products is crucial patients. Reliable methods cell purification are very important commercialization those new therapies. With large production scale envisioned commercialization, isolation need to be efficient, robust, operationally simple and generic while ensuring biological functionality safety. In this study, we high magnetized magnetic agarose-based beads conjugated with protein A develop a method separation. separation efficiency 91% yield consistent performances were demonstrated using population mixtures human mesenchymal stem HER2+ SKBR3 (80:20, 70:30 30:70). Additionally, robustness against mechanical stress minimal unspecific binding obtained base significant advantages in comparison same microparticles where antibodies covalently conjugated. This study provided insights on features microparticles, which promising large-scale application purification.