Promoting Nerve Cell Functions on Hydrogels Grafted with Poly(l-lysine)

Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs

Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid) hydrogels with optimal elasticity, and prepare...

Immobilization of Glucose Oxidase on a Novel Crosslinked Chitosan Support Grafted with L-Lysine Spacers

A novel L-lysine modified semi-crosslinked chitosan resin (LMCCR) was synthesized and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidases (GODs) immobilized on it. The prepared LMCCR beads have large specific surface and excellent chemical stability. The insertion of flexible L-lysine spacers between chitosan backbone and the immobilized GODs (...

Promoting Extracellular Matrix Crosslinking in Synthetic Hydrogels

Modeling the Swelling Pressure of Degrading Hydroxyethylmethacrylate-Grafted Dextran Hydrogels

Degrading hydroxyethylmethacrylate-grafted dextran (dex-HEMA) hydrogels generate a relatively sudden increase in osmotic pressure upon degradation into dextran solutions. This phenomenon is currently being examined as a possible means of developing a pulsatile drug-delivery system. Here a mathematical model based on scaling concepts is presented to describe this sudden increase in swelling pres...

Evaluation of the Effect of Psyllium on the Viability of Lactobacillus Acidophilus in Alginate-Polyl Lysine Beads.

Purpose: Psylliumseeds are used in traditional herbal medicine to treat various disorders. Moreover, as a soluble fiber, psyllium has potential to stimulate bacterial growth in digestive system. We aimed to substitute alkali-extractable polysaccharides of psyllium for alginate in beads with second coat of poly-l-lysine to coat Lactobacillus acidophilus. Methods: Beads were prepared using extrus...