Prokaryotic iron superoxide dismutase replaces cytosolic copper, zinc superoxide dismutase in protecting yeast cells against oxidative stress

Cytosolic Superoxide Dismutase (SOD1) Is Critical for Tolerating the Oxidative Stress of Zinc Deficiency in Yeast

Zinc deficiency causes oxidative stress in many organisms including the yeast Saccharomyces cerevisiae. Previous studies of this yeast indicated that the Tsa1 peroxiredoxin is required for optimal growth in low zinc because of its role in degrading H(2)O(2). In this report, we assessed the importance of other antioxidant genes to zinc-limited growth. Our results indicated that the cytosolic sup...

Copper-zinc superoxide dismutase from the marine yeast Debaryomyces hansenii.

We have isolated the cytosolic form of Cu-Zn superoxide dismutase (SOD) from the marine yeast Debaryomyces hansenii. This enzyme has a subunit mass of 18 kDa. The preparation was found to be heterogeneous by IF electrophoresis with two pI ranges: 5.14-4.0 and 1.6-1.8. The enzyme preparation had a remarkably strong stability at pH 6.0-7.0, surviving boiling for 10 min without losing more than 60...

Total reconstitution of copper-zinc superoxide dismutase.

The total reconstitution of copper-zinc superoxide dismutase by stoichiometric addition of the metals to completely metal-free apoprotein is described. Reconstitution has been monitored by electron paramagnetic resonance (EPR) and absorption spectroscopy, enzymic activity, and acrylamide gel el%ctrophoresis. Under the same conditions, it has been possible to prepare highly active enzymes with c...

Superoxide dismutase Superoxide dismutase

Oxidative stress and superoxide dismutase in schizophrenia.

Oxyradicals are chemical species with an unpaired electron in one of their orbits. Production of oxyradicals occurs throughout the body during cellular metabolism [l]. A complex antioxidant system exists to regulate levels of oxyradicals as excessive production or ineffective removal can result in cellular damage. The antioxidant enzymes present indude superoxide dismutase (SOD), glutathione pg...