Progressive Motility, DNA Fragmentation, Intact Plasma Membrane, and Acrosome Status of Frozen Semen Bali and Simmental Bulls

In increasing the population of bulls in Indonesia, it is necessary to utilize Artificial Insemination (AI) technology. AI reproductive technology aims increase efficiency and use superior semen livestock can prevent spread diseases. Frozen one factors that influence success AI. Thus, quality frozen must be maintained so fertility remains good. This study aimed determine Bali Simmental at Center, South Sulawesi. used from two bulls. Parameters observed were progressive motility, deoxyribonucleic acid fragmentation (DNA), acrosome status, intact plasma membrane. The motility was tested using Computer-assisted sperm analysis (CASA Sperm Vision Minitube), DNA acridine orange dye, status peanut agglutinin, membrane staining HOS solution. gives a result fragmentation, spermatozoa not significantly different (P>0.05). contrast, whole bulls' higher (P<0.05) than results indicate based on parameters spermatozoa, both bull breeds had same semen. Meanwhile, according membrane, better Keywords: Insemination, quality,