Production, purification and crystallization of an alkaline protease from Aspergillus tamarii[EF661565.1]
نویسندگان
چکیده
منابع مشابه
Purification, characterization and crystallization of an extracellular alkaline protease from Aspergillus nidulans HA-10.
Aspergillus nidulans is a highly potent fungus used in the production of alkaline protease. Extracellular alkaline protease was purified from A. nidulans in a two-step procedure involving ammonium sulphate precipitation and Sephadex G-100 column chromatography. The molecular mass of the enzyme was determined to be 42 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelati...
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The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
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Background: Alkaline proteases is the important group of enzymes having numerous industrial applications including dairy food formulations. Objectives: The current study deals with the purification and characterization of an alkaline serine protease produced by Geotrichum candidum QAUGC01, isolated from indigenous fermented milk product, Dahi.<br...
متن کاملProduction, Purification and Characterization of an Alkaline Protease from an Alkaliphilic Bacillus Sp
An extracellular serine like alkaline protease was purified and characterized from alkaliphilic Bacillus sp. The enzyme was purified to the homogeneity by a combination of aqueous two-phase system and affinity chromatographic techniques. The apparent molecular weight was estimated as 26-29 kDa based on SDS-PAGE and gel filtration chromatography and the enzyme appeared to be a monomer. The Km an...
متن کاملIsolation, Production, Purification, Assay and Characterization of Alkaline Protease Enzyme from Aspergillus niger and its Compatibility with Commercial Detergents
Aspergillus niger is a highly potent fungus used in the production of alkaline protease. Extra cellular alkaline protease was purified from A. niger in a twostep procedure involving ammonium sulphate precipitation and Sephadex G100 column chromatography. The molecular mass of the enzyme was determined to be 60 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelatin. The ...
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ژورنال
عنوان ژورنال: Agriculture and Biology Journal of North America
سال: 2011
ISSN: 2151-7517,2151-7525
DOI: 10.5251/abjna.2011.2.7.1135.1142