Production, purification and characterization of thermostable alpha amylase from Bacillus subtilis Y25 isolated from decaying yam (Dioscorea rotundata) tuber
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چکیده
منابع مشابه
Purification and Characterization of a Novel Thermostable and Acid Stable α-Amylase from Bacillus Sp. Iranian S1
This study reports the purification and biochemical characterization of thermostable and acidic-pH-stable α-amylase from Bacillus sp. Iranian S1 isolated from the desert soil (Gandom-e-Beryan in Lut desert, Iran). Amylase production was found to be growth associated. Maximum enzyme production was in exponential phase with activity 2.93 U ml-1 at 50°C and pH 5. The enzyme was purified by isoprop...
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Pectinases are industrially important enzymes. The aim of this work was the isolation of pectinases producing Bacillus species from soil sample and their identification by phenotypic tests as well as production and purification pectinases enzyme. Isolated bacteria Subjected to various screening activity and gives pectinases screening test positive i.e. bacteria produced pectinases enzyme. The e...
متن کاملProduction and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation
Amylase is an enzyme that catalyzes the breakdown of starch into sugars and plays a pivotal role in a variety of areas like use as digestives, for the production of ethanol and high fructose corn syrup, detergents, desiring of textiles, modified starches, hydrolysis of oil-field drilling fluids, and paper recycling. In the present work, solid state fermentation (SSF) for α -amylase production h...
متن کاملPartial characterization and optimization of production of extracellular alpha-amylase from Bacillus subtilis isolated from culturable cow dung microflora.
Studies of alpha-amylase production by Bacillus subtilis (CM3) isolated earlier from cow dung microflora, were carried out. The optimum temperature, pH and incubation period for amylase production were 50-70 degrees C, 5.0-9.0 and 36 h, respectively. Enzyme secretion was very similar in the presence of any of the carbon sources tested (soluble starch, potato starch, cassava starch, wheat flour,...
متن کاملIndole-3-acetic acid production and effect on sprouting of yam (Dioscorea rotundata L.) minisetts by Bacillus subtilis isolated from culturable cowdung microflora.
Bacillus subtilis strains (CM1-CM5) isolated from culturable cowdung microflora were investigated for indole-3-acetic acid (IAA) production in nutrient broth (NB). All the strains tested produced IAA in NB; albeit in very low concentrations (0.09-0.37 mg/l). The addition of L-tryptophan (0.1 - 1.0 g/l) into NB substantially enhanced IAA production (6.1 - 31.5 folds) indicating that L-tryptophan...
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ژورنال
عنوان ژورنال: Notulae Scientia Biologicae
سال: 2020
ISSN: 2067-3264,2067-3205
DOI: 10.15835/nsb12110521