Production, Purification and Characterization of Extracellular Lipase from a Mutated Strain of Penicillium Citrinum KU613360
نویسندگان
چکیده
منابع مشابه
Purification of endoglucanase produced by Penicillium citrinum isolated from Amazon
Background The cellulolytic enzyme complex has many important biotechnological applications such as beverage, textile, food, paper and cellulose industries, as well as in degradation of lignocellulosic for ethanol biofuel production [1]. According to the enzymatic activity, cellulolytic complex is subdivided in three classes: endoglucanases, exoglucanases and b-D-glycosidases. Endoglucanases (e...
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An extra cellular lipase was isolated and purified from the culture broth of Pseudomonas aeruginosa SRT 9 to apparent homogeneity using ammonium sulfate precipitation followed by chromatographic techniques on phenyl Sepharose CL- 4B and Mono Q HR 5/5 column, resulting in a purification factor of 98 fold with specific activity of 12307.8 U/mg. The molecular weight of the purified lipase was esti...
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Psychrotrophic Pseudomonas ADT3 (NCBI GenBank Acc.no.JX914667) is capable of growth on lipid as the sole carbon source. In this paper, we report the purification and characterization of an extracellular lipase from psychrotroph, isolated from soil sample of NyAlesund, Svalbard, Arctic region. The Pseudomonas ADT3 isolate produces lipase enzyme in the extracellular minimal media with only 1% oli...
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The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/ mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50oC, and were stable betwee...
متن کاملProduction and characterization of an extracellular lipase from Candida guilliermondii
Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and pr...
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ژورنال
عنوان ژورنال: International Journal of Scientific Research in Science and Technology
سال: 2021
ISSN: 2395-602X,2395-6011
DOI: 10.32628/ijsrst207697