Production of xylo-oligosaccharides by immobilized-stabilized derivatives of endo-xylanase from Streptomyces halstedii
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منابع مشابه
PRODUCTION OF XYLO-OLIGOSACCHARIDES FROM MISCANTHUS x GIGANTEUS BY AUTOHYDROLYSIS
Xylo-oligosaccharides were obtained from Miscanthus x giganteus. The process was designed as a biorefinery scheme, which seeks the separation of the three main components: cellulose, hemicelluloses, and lignin. To extract the hemicelluloses, particularly xylans, in an efficient way, Miscanthus was subjected to autohydrolysis. The system was evaluated for the effects of temperature (160 to 200oC...
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Objective(s): Although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed. Materials and Methods: Single xylanase producer yeast was isolated from plant residues based on formation of transparent halo zones on xylan agar plates. The isolate showed much ...
متن کاملGlycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi.
Glycosynthases are synthetic enzymes derived from retaining glycosidases in which the catalytic nucleophile has been replaced. The mutation allows irreversible glycosylation of sugar acceptors using glycosyl fluoride donors to afford oligosaccharides without any enzymatic hydrolysis. Glycosynthase technology has proven fruitful for the facile synthesis of useful oligosaccharides, therefore the ...
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Background: The extracellular xylanase secreted by microorganisms is a hydrolytic enzyme, which arbitrarily cleaves the β-1, 4 backbone of the polysaccharide xylan; an enzyme used in the food processing, bio-pulping and bio-bleaching. The commercial production of the xylanase is limited because of a higher cost involvement, which can be overcome by the cost-effective production...
متن کاملProduction of Xylo-oligosaccharides by Chemo- Enzymatic Treatment of Agricultural By-products
Several timbers and crop by-products were subjected to an enzymatic treatment to obtain a xylo-oligosaccharides-enriched preparation. The process was performed by means of the commercial endo-β-1,4xylanase Buzyme 2511. The enzymatic cocktail was applied onto the raw ground materials with yield up to 5.3 ± 1.0 g/L xylo-oligosaccharides for apple pomace. In order to make the materials more access...
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ژورنال
عنوان ژورنال: Process Biochemistry
سال: 2013
ISSN: 1359-5113
DOI: 10.1016/j.procbio.2013.01.010