Production of viable trout offspring derived from frozen whole fish
نویسندگان
چکیده
منابع مشابه
Production of viable trout offspring derived from frozen whole fish
Long-term preservation of fish fertility is essential for the conservation of endangered fishes. However, cryopreservation techniques for fish oocytes and embryos have not yet been developed. In the present study, functional eggs and sperm were derived from whole rainbow trout that had been frozen in a freezer and stored without the aid of exogenous cryoprotectants. Type A spermatogonia retriev...
متن کاملSpermatogonial Transplantation in Fish: Production of Trout Offspring from Salmon Parents
Many salmonid species have recently become at risk of extinction. For fish species where eggs cannot be cryopreserved, establishment of techniques to preserve genetic resources other than egg and embryo cryopreservation is imperative. In the present study, spermatogonia from male trout were transplanted into the peritoneal cavity of newly hatched sterile triploid salmon. Transplanted trout sper...
متن کاملSpermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring.
Cryopreservation of male germ cells is a strategy to conserve animal species and strains of animals valuable to biomedical research. We tested whether mouse male germ cells could be cryopreserved without cryoprotection by simply freezing epididymides, testes, or whole bodies. The reproductive organs were isolated from killed mice and frozen for 1 week to 1 year at -80 degrees C before spermatoz...
متن کاملViable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads.
A 2 x 3 factorial designed experiment was conducted in order to examine whether freeze-dried rat spermatozoa can participate in full-term development following intracytoplasmic sperm injection (ICSI). A sperm suspension from cauda epididymides of Sprague-Dawley (SD) rats was prepared with or without ultrasonic treatment. The sonicated and non-sonicated sperm suspensions were processed for freez...
متن کامل"Viable Offspring Derived from Fetal and Adult Mammalian Cells" (1997), by Ian Wilmut et al
In the 1990s, Ian Wilmut [3], Jim McWhir, and Keith Campbell performed experiments while working at the Roslin Institute in Roslin, Scotland. Wilmut, McWhir, and Campbell collaborated with Angelica Schnieke and Alex J. Kind at PPL Therapeutics in Roslin, a company researching cloning [4] and genetic manipulation for livestock. Their experiments resulted in several sheep [5] being born in July 1...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2015
ISSN: 2045-2322
DOI: 10.1038/srep16045