Production of the antibiotic FR-008/candicidin in Streptomyces sp. FR-008 is co-regulated by two regulators, FscRI and FscRIV, from different transcription factor families
نویسندگان
چکیده
منابع مشابه
Development of Streptomyces sp. FR-008 as an emerging chassis☆
Microbial-derived natural products are important in both the pharmaceutical industry and academic research. As the metabolic potential of original producer especially Streptomyces is often limited by slow growth rate, complicated cultivation profile, and unfeasible genetic manipulation, so exploring a Streptomyces as a super industrial chassis is valuable and urgent. Streptomyces sp. FR-008 is ...
متن کاملarsRBOCT arsenic resistance system encoded by linear plasmid pHZ227 in Streptomyces sp. strain FR-008.
In the arsenic resistance gene cluster from the large linear plasmid pHZ227, two novel genes, arsO (for a putative flavin-binding monooxygenase) and arsT (for a putative thioredoxin reductase), were coactivated and cotranscribed with arsR1-arsB and arsC, respectively. Deletion of the ars gene cluster on pHZ227 in Streptomyces sp. strain FR-008 resulted in sensitivity to arsenic, and heterologou...
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Background: Vascular endothelial growth factor (VEGF) has a key role in angiogene-sis and in transplantation. The level of VEGF is related to the differences in the DNA sequence of its promoter region. Objectives: In this study, the association between the combination of VEGF –1154 G and –2578 C alleles and VEGF production by LPS-stimulated PBMCs was investigated. In addition; the relationship ...
متن کاملPrecursors in the biosynthesis of FR-900482, a novel antitumor antibiotic produced by Streptomyces sandaensis.
isolated from the culture broth of Streptomyces sandaensis No. 6897, was found to have unique structure1*^. FR-900482 differs from the mitomycin family of antibiotics due to the lack of a quinoid structure, although FR-900482 resembles the mitomycins with respect to the aziridine function and a carbamoyloxymethyl group. Examination of the structure suggests that the biosynthesis of FR-900482 ma...
متن کاملTailoring the P450 monooxygenase gene for FR-008/candicidin biosynthesis.
Tailoring steps are often important for the activity of mature antibiotics. Here, we report that novel decarboxylated FR-008/candicidin derivatives were obtained from the P450 monooxygenase gene fscP mutant of Streptomyces sp. strain FR-008. The toxicity of decarboxylated FR-008/candicidin derivatives has been shown to be greatly reduced compared to that of wild-type FR-008/candicidin.
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ژورنال
عنوان ژورنال: Microbiology
سال: 2015
ISSN: 1350-0872,1465-2080
DOI: 10.1099/mic.0.000033