PRODUCTION OF PROSTAGLANDIN F BY RABBIT GRANULOSA CELLS AND THECAL TISSUE
نویسندگان
چکیده
منابع مشابه
Role of mitogen-activated protein kinase in prostaglandin f(2alpha) action in human granulosa-luteal cells.
In the ovary it has been demonstrated that PGF(2alpha) activates the phospholipase C (PLC)/diacylglycerol/protein kinase C pathway. However, little is known about the downstream signaling events that mediate subsequent cellular responses such as steroidogenesis. The present study was designed to examine the effect of PGF(2alpha) on activation of the mitogen-activated protein kinase (MAPK) signa...
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Link protein (LP), an extracellular matrix protein in cartilage, stabilizes aggregates of hyaluronic acid (HA) and proteoglycans, including aggrecan and inter-alpha-trypsin inhibitor (ITI). We have shown previously that cartilage LP is present in the maturing rat and mouse ovary. In the present study, we have employed immunohistochemistry to examine the anatomical distribution of cartilage LP i...
متن کاملOptimized primary culture and subculture of granulosa cells
Background: Primordial follicle includes an oocyte surrounded by a layer of somatic cells called granulosa cells (GCs). GCs, also known as nurse cells, are an important protective element for the growth and survival of oocytes. Oocytes, which lack some of the metabolic processes required granulosa cells for their development. Objective: This manuscript has been provided to explain the protocol...
متن کاملProstaglandin biosynthesis by rabbit renomedullary interstitial cells in tissue culture. Stimulation by angiotensin II, bradykinin, and arginine vasopressin.
Rabbit renomedullary interstitial cells were isolated and grown in tissue culture. These cells synthesize 0.8 ng of prostaglandin E2 (PGE2) per microgram cellular protein per hour in monolayer tissue culture; prostaglandins A2 and F2alpha (PGA2 and PGF2alpha) biosynthesis was 10 and 5% of PGE2 biosynthesis, respectively. Arachidonic acid markedly stimulated the production of PGE2 and PGF2alpha,...
متن کامل4-Aminopyridine Decreases Progesterone Production by Porcine Granulosa Cells
BACKGROUND Ion channels occur as large families of related genes with cell-specific expression patterns. Granulosa cells have been shown to express voltage-gated potassium channels from more than one family. The purpose of this study was to determine the effects of 4-aminopyridine (4-AP), an antagonist of KCNA but not KCNQ channels. METHODS Granulosa cells were isolated from pig follicles and...
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ژورنال
عنوان ژورنال: Obstetrical & Gynecological Survey
سال: 1977
ISSN: 0029-7828
DOI: 10.1097/00006254-197706000-00016