Production of Neurospora mycelial protoplasts
نویسندگان
چکیده
منابع مشابه
Protoplasts from Neurospora crassa.
Weibull (1953) demonstrated that the intact protoplasts are liberated from cells of Bacillus megaterium after digestion of the cell wall by the enzyme lysozyme, and that these structures persist and exhibit many of the activities of the whole cell provided they are maintained in solutions of appropriate osmotic pressure. Since that time free protoplasts have been obtained from many other gram-p...
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Both mycelial fragments and protoplasts were successfully employed for mutagenesis of Micromonospora rosaria NRRL 3718, and the results were compared. The optimal conditions and effective procedures for mutagenesis of M. rosaria by a chemical mutagen, N-methyl-N'-nitro-N-nitrosoguanidine, have been determined. Mutation was efficiently induced when mycelial fragments were treated with N-methyl-N...
متن کاملEffect of polyene antibiotics on protoplasts of Neurospora crassa.
Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Effect of polyene antibiotics on protoplasts of Neurospora crassa. J. Bacteriol. 83:351-358. 1962.-The polyene antibiotics nystatin, amphotericin B, and filipin induced shrinkage of Neurospora crassa protoplasts stabilized in sucrose. At low concentrations of filipin (5 mug per ml), or higher concentrations of nystatin and amphotericin...
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Protoplasts, Soybean Cell Suspension Cultures, Phytoalexin Production, Phytophthora megasperm a f. sp. glycinea, Glucan Elicitor Protoplasts isolated enzymatically from suspension-cultured cells of soybean (Glycine max L. Merr., cv. Harosoy 63) were used to study the production of the isoflavonoid-derived phytoale xin, glyceollin. A large enhancement in the in vivo rates of synthesis and catal...
متن کاملExpressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa.
In the Neurospora Genome Project at the University of New Mexico, expressed sequence tags (ESTs) corresponding to three stages of the life cycle of the filamentous fungus Neurospora crassa are being analyzed. The results of a pilot project to identify expressed genes and determine their patterns of expression are presented. 1,865 partial complementary DNA (cDNA) sequences for 1,409 clones were ...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1967
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1963