Production of Influenza Virus Proteins Using Recombinant Insect Cells

Influenza vaccines have long been manufactured in embryonated chicken eggs. This method has some problems such as a production period (about 6 months) and use of large amounts infectious pathogens. Recently, the recombinant subunit using baculovirus–insect cell system extensively investigated. In this system, viral immunodominant components can be produced more rapidly larger scale than conventional egg-based process. However, continuous is virtually impossible because infection baculovirus results death host insect cells. present study, we established stably transformed cells that secreted influenza virus-like particles (VLPs) consisting hemagglutinin (HA), major protective antigen A virus, matrix protein 1 (M1), another structural virus. Hemagglutination assay transmission electron microscopy (TEM) suggested HA by kept hemagglutination activity morphology VLPs was similar to wild type virus particles.