Production of infectious hepatitis C virus in cell culture
نویسندگان
چکیده
منابع مشابه
Production of Infectious Hepatitis C Virus of Various Genotypes in Cell Culture Running Title; Infectious HCV Production in Cell Culture
A unique hepatitis C virus (HCV) strain JFH-1 has been shown to replicate efficiently in cell culture with production of infectious HCV. We previously developed a DNA expression system containing HCV cDNA flanked by two self-cleaving ribozymes to generate HCV particles in cell culture. In this study, we produced HCV particles of various genotypes including 1a (H77), 1b (CG1b) and 2a (J6 and JFH...
متن کاملOvercoming Hurdles in Hepatitis C Virus Research: Efficient Production of Infectious Virus in Cell Culture
Hepatitis C virus is a flavivirus that infects nearly 2% of the world population. There is no vaccine available and current therapy with interferon and ribavirin is expensive, not well tolerated and effective in only 60% of patients. HCV research has been hampered by the lack of a robust tissue culture system, but recent advances have made virus growth in culture possible. Here we review the cu...
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متن کاملCell culture systems for hepatitis C virus.
Due to the obligatory intracellular lifestyle of viruses, cell culture systems for efficient viral propagation are crucial to obtain a detailed understanding of the virus-host cell interaction. For hepatitis C virus (HCV) the development of permissive and authentic culture models continues to be a challenging task. The first efforts to culture HCV had limited success and range back to before th...
متن کاملCell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro.
Hepatitis C virus (HCV) is a major cause of chronic liver disease, frequently progressing to cirrhosis and increased risk of hepatocellular carcinoma. Current therapies are inadequate and progress in the field has been hampered by the lack of efficient HCV culture systems. By using a recently described HCV genotype 2a infectious clone that replicates and produces infectious virus in cell cultur...
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ژورنال
عنوان ژورنال: Uirusu
سال: 2005
ISSN: 0042-6857
DOI: 10.2222/jsv.55.287