Production of galactooligosaccharides with .BETA.-galactosidase.
نویسندگان
چکیده
منابع مشابه
Continuous Packed Bed Reactor with Immobilized -Galactosidase for Production of Galactooligosaccharides (GOS)
The β-galactosidase from Bacillus circulans was covalently attached to aldehyde-activated (glyoxal) agarose beads and assayed for the continuous production of galactooligosaccharides (GOS) in a packed-bed reactor (PBR). The immobilization was fast (1 h) and the activity of the resulting biocatalyst was 97.4 U/g measured with o-nitrophenyl-β-D-galactopyranoside (ONPG). The biocatalyst showed exc...
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Different compositions of liquid media were used for their efficacy in the production of beta-galactosidase by Aspergillus oryzae in submerged fermentation. Enzyme production was improved by switching from lactose-based media to soybean meal- and wheat bran-based media. Further improvement was made when the soybean meal medium was supplemented with sodium nitrate and magnesium sulfate. Res...
متن کاملProduction of beta-galactosidase from Streptococcus thermophilus grown in whey.
beta-D-Galactosidase (EC 3.2.1.23) was extracted from Streptococcus thermophilus grown in deproteinized cheese whey. Cultural conditions optimum for maximum enzyme production were pH 7.0, 40 degrees C, and 24 h. Proteose peptone (2.0%, wt/vol) and corn steep liquor (2.8%, wt/vol) were highly stimulatory, increasing the enzyme units available in their absence from 660 U/liter of medium to 18,200...
متن کاملproduction of beta-galactosidase in submerged media by aspergillus oryzae, ptcc 5 163
different compositions of liquid media were used for their efficacy in the production of beta-galactosidase by aspergillus oryzae in submerged fermentation. enzyme production was improved by switching from lactose-based media to soybean meal- and wheat bran-based media. further improvement was made when the soybean meal medium was supplemented with sodium nitrate and magnesium sulfate. results ...
متن کاملProduction of Galactooligosaccharides Using β-Galactosidase Immobilized on Chitosan-Coated Magnetic Nanoparticles with Tris(hydroxymethyl)phosphine as an Optional Coupling Agent
β-Galactosidase was immobilized on chitosan-coated magnetic Fe3O4 nanoparticles and was used to produce galactooligosaccharides (GOS) from lactose. Immobilized enzyme was prepared with or without the coupling agent, tris(hydroxymethyl)phosphine (THP). The two immobilized systems and the free enzyme achieved their maximum activity at pH 6.0 with an optimal temperature of 50 °C. The immobilized e...
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ژورنال
عنوان ژورنال: Journal of the Japanese Society of Starch Science
سال: 1989
ISSN: 1884-488X,0021-5406
DOI: 10.5458/jag1972.36.123