Production of a Cephalosporinase by Pseudomonas pyocyanea

Cephalosporinase and penicillinase activities of a beta-lactamase from Pseudomonas pyocyanea.

1. Pseudomonas pyocyanea N.C.T.C. 8203 produces a beta-lactamase that is inducible by high concentrations of benzylpenicillin or cephalosporin C. Methicillin appeared to be a relatively poor inducer, but this could be attributed in part to its ability to mask the enzyme produced. Much of the enzyme is normally cell-bound. 2. No evidence was obtained that the crude enzyme preparation consisted o...

A Bacteriophage for Pseudomonas pyocyanea (Pseudomonas aeruginosa).

Improved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant

A Pseudomonas aeruginosa mutant derived by random mutagenesis with N-methyl-N¢-nitro-N-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on Siegmund-Wagner (SW) plates. The mutant designated P. aeruginosa PTCC1637 produces rhamnolipids at concentrations 10 time more than parent strain. NMR analysis and surface tension measurement showed that the biosurfactan...

Pseudomonas Pyocyanea and the Arginine Dihydrolase System.

Non-pigmented strains of Pseudomonas pyocyanea occur frequently and this organism has only limited activity in conventional biochemical tests; 50 strains were tested for the presence of arginine dihydrolase and found positive whereas only Salmonella sp. and Enterobacter sp. among other Gram-negative species were positive. The test for arginine dihydrolase is rapid and simple and suitable for ro...

Incubator-borne Pseudomonas pyocyanea infection in a newborn nursery.

In recent years, Pseudomonas pyocyanea and other Gram-negative bacteria have appeared more frequently as important pathogens. This has followed the effective antibiotic treatment of infections due to most other organisms and is also related to the increasing use of certain equipment which may become readily contaminated and which is difficult to sterilize. The problem is particularly serious in...