Production of 15N-labeled α-amanitin in Galerina marginata
نویسندگان
چکیده
منابع مشابه
Fermentative production of amanita toxins by a strain of Galerina marginata.
Among the violently poisonous species of Amanita, none has received more attention than A. phalloides (Fr.) Secr. By 1959, five related cyclopeptidic toxins had been extracted from this mushroom and named phalloidin, phalloin, and a-, j3-, and y-amanitin. T. Wieland and 0. Wieland (Pharmacol. Rev. 11:87, 1959) reviewed the chemistry and toxicology of these compounds. A sixth toxin, phallacidin,...
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Species in the mushroom genus Lepiota can cause fatal mushroom poisonings due to their content of amatoxins such as α-amanitin. Previous studies of the toxin composition of poisonous Lepiota species relied on analytical methods of low sensitivity or resolution. Using liquid chromatography coupled to UV absorbance and mass spectrometry, we analyzed the spectrum of peptide toxins present in six I...
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15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and neutral pH but quite pronounced changes in proton-15N nuclear Overhauser effe...
متن کاملSyntheses of 15N-labeled pre-queuosine nucleobase derivatives
Pre-queuosine or queuine (preQ1) is a guanine derivative that is involved in the biosynthetic pathway of the hypermodified tRNA nucleoside queuosine (Que). The core structure of preQ1 is represented by 7-(aminomethyl)-7-deazaguanine (preQ1 base). Here, we report the synthesis of three preQ1 base derivatives with complementary (15)N-labeling patterns, utilizing [(15)N]-KCN, [(15)N]-phthalimide, ...
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ژورنال
عنوان ژورنال: Toxicon
سال: 2015
ISSN: 0041-0101
DOI: 10.1016/j.toxicon.2015.06.018