Production and Characterization of Immunoglobuline Yolk as anti antigen membrane Toxoplasma gondii

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Production of MAG1 Antigen of Toxoplasma gondii in Escherichia coli

  Introduction : Toxoplasmosis is a parasitic infection caused by the protozoan Toxoplasma gondii it leads to serious medical problems in congenitally-infected and immunocompromised individuals, while it is quite harmless in immunocompetent individuals. Toxoplasma tissue cyst matrix protein (MAG1) induces early humoral and cell-mediated immune responses. Previous studies suggested recombinant M...

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Bacterial Production of Dense Granule Antigen GRA8 of Toxoplasma gondii

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Production, Characterization and Applications for Toxoplasma gondii-Specific Polyclonal Chicken Egg Yolk Immunoglobulins

BACKGROUND Toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. Immunized mammals are a wide source of hyperimmune sera used in different approaches, including diagnosis and the study of host-parasite interactions. Unfortunately, mammalian antibodies present limitations for its production, such as the necessity for animal bleeding, low yield, interferenc...

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Bacterial production of dense granule antigen GRA8 of Toxoplasma gondii.

BACKGROUND Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. METHODS GRA8 comple...

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Production of MAG1 Antigen of Toxoplasma gondii in Escherichia coli

Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect human and other warm-blooded animals. Although usually asymptomatic in immunocompetent individuals, toxoplasmosis may reactivate in immunocompromised patients (e.g. AIDS patients and organ transplant recipients) and cause severe diseases such as toxoplasmic encephalitis. Moreover, primary maternal infection can pu...

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ژورنال

عنوان ژورنال: Indonesian Journal of Tropical and Infectious Disease

سال: 2016

ISSN: 2356-0991,2085-1103

DOI: 10.20473/ijtid.v6i2.1365