Producing Purer Podocytes

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Evolutionary morphology of podocytes and primary urine-producing apparatus

Excretory organs were acquired in the early phase of metazoan evolution, and they play a crucial role in the maintenance of homeostasis of body fluids. In general, these organs consist of two functional components, the primary-urine producing apparatus and the modulating tubule. This basic organization of the excretory organs is conserved among most metazoans. Herein, we present an overview of ...

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Bioenergetic characterization of mouse podocytes.

Mitochondrial dysfunction contributes to podocyte injury, but normal podocyte bioenergetics have not been characterized. We measured oxygen consumption rates (OCR) and extracellular acidification rates (ECAR), using a transformed mouse podocyte cell line and the Seahorse Bioscience XF24 Extracellular Flux Analyzer. Basal OCR and ECAR were 55.2 +/- 9.9 pmol/min and 3.1 +/- 1.9 milli-pH units/min...

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Podocytes as a therapeutic target

The filtration system of a kidney is critical for retaining essential proteins from the blood plasma and removal of toxic waste from the body. When a kidney loses its filtration function it results in life threatening complications and the survival usually depends on dialysis and eventually surgical intervention requiring a kidney transplant. The filtration function in a kidney is carried out b...

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Stressed-out podocytes in diabetes?

Diabetic nephropathy is the most common cause of ESRD in the United States.1 Although current strategies can slow disease progression,2,3 development of renal failure requiring renal replacement therapy is a distressingly common outcome in patients with diabetes.1 As a result, much effort has been devoted to understanding the mechanisms that promote glomerular damage in diabetic kidney disease ...

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Amino acid transport in podocytes.

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ژورنال

عنوان ژورنال: Journal of the American Society of Nephrology

سال: 2019

ISSN: 1046-6673,1533-3450

DOI: 10.1681/asn.2018101045