Probing and quantifying DNA–protein interactions with asymmetrical flow field-flow fractionation
نویسندگان
چکیده
منابع مشابه
Miniaturization of frit inlet asymmetrical flow field-flow fractionation.
A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of sample materials. The end effect contribut...
متن کاملStopless flow injection in asymmetrical flow field-flow fractionation using a frit inlet.
Stopless flow operation of asymmetrical flow field-flow fractionation (FFF) has been achieved by introducing a hydrodynamic relaxation method using a frit inlet. By using frit inlet injection, a focusing process which has been an essential part of runs at the asymmetrical flow FFF system can be completely avoided. Band broadening of an initial sample zone during hydrodynamic relaxation is discu...
متن کاملNanoparticle separation with a miniaturized asymmetrical flow field-flow fractionation cartridge
Asymmetrical Flow Field-Flow Fractionation (AF4) is a separation technique applicable to particles over a wide size range. Despite the many advantages of AF4, its adoption in routine particle analysis is somewhat limited by the large footprint of currently available separation cartridges, extended analysis times and significant solvent consumption. To address these issues, we describe the fabri...
متن کاملComparison of Miniaturized and Conventional Asymmetrical Flow Field-Flow Fractionation (AF4) Channels for Nanoparticle Separations
The performance of a miniaturized channel for the separation of polymer and metal nanoparticles (NP) using Asymmetrical Flow Field-Flow Fractionation (AF4) was investigated and compared with a conventional AF4 system. To develop standard separation methods, experimental parameters like cross flow, gradient profile and injection time were varied and optimized. Corresponding chromatographic param...
متن کاملEffect of asymmetrical flow field-flow fractionation channel geometry on separation efficiency.
The separation efficiencies of three different asymmetrical flow field-flow fractionation (AF4) channel designs were evaluated using polystyrene latex standards. Channel breadth was held constant for one channel (rectangular profile), and was reduced either linearly (trapezoidal profile) or exponentially (exponential profile) along the length for the other two. The effective void volumes of the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2014
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2014.07.002