Primers for Castilleja and their utility across Orobanchaceae: I. Chloroplast primers 1
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چکیده
منابع مشابه
Primers for Castilleja and their utility across Orobanchaceae: I. Chloroplast primers1
PREMISE OF THE STUDY Chloroplast primers were developed from genomic data for the taxonomically challenging genus Castilleja. We further tested the broader utility of these primers across Orobanchaceae, identifying a core set of chloroplast primers amplifying across the clade. METHODS AND RESULTS Using a combination of three low-coverage Castilleja genomes and sequence data from 12 Castilleja...
متن کاملPrimers for Castilleja and their utility across Orobanchaceae: II. Single-copy nuclear loci1
PREMISE OF THE STUDY We developed primers targeting nuclear loci in Castilleja with the goal of reconstructing the evolutionary history of this challenging clade. These primers were tested across other major clades in Orobanchaceae to assess their broader utility. METHODS AND RESULTS We assembled low-coverage genomes for three taxa in Castilleja and developed primer combinations for the singl...
متن کاملPanero and Crozier: Asteraceae Chloroplast Dna Primers Primers for Pcr Amplification of Asteraceae
Primers for the amplification and sequencing of DNA fragments from chloroplast genes and non-coding regions are provided to facilitate molecular phylogenetic studies aimed at building a tree-of-life for the Asteraceae. The primers reported here have been extensively tested and some empirical guidelines are included to facilitate their use.
متن کاملCharacterization of microsatellite loci in Castilleja sessiliflora and transferability to 24 Castilleja species (Orobanchaceae)1
UNLABELLED PREMISE OF THE STUDY Microsatellite primers were developed in the hemiparasitic perennial forb Castilleja sessiliflora to investigate patterns of gene flow and genetic diversity within and among populations. • METHODS AND RESULTS Twelve polymorphic loci were identified in C. sessiliflora and tested on three populations (32 individuals each) sampled across the range of the specie...
متن کاملTangled trios?: Characterizing a hybrid zone in Castilleja (Orobanchaceae).
Hybridization and polyploidization are exceedingly important processes because both influence the ecological envelope and evolutionary trajectory of land plants. These processes are frequently invoked for Castilleja (Indian paintbrushes) as contributors to morphological and genetic novelty and as complicating factors in species delimitations. Here, we provide a detailed analysis of morphologica...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2017
ISSN: 2168-0450,2168-0450
DOI: 10.3732/apps.1700020