Prime Editing: Precision Genome Editing by Reverse Transcription

Genome Editing



HIV-1 RNA editing, hypermutation, and error-prone reverse transcription.

Bourara et al. (1) reported that transcripts of the human immunodeficiency virus–type 1 (HIV-1) were subject to RNA editing in chronically infected cells. They observed multiple guanine-to-adenine (G-to-A) and cytosine-to-uracil (C-to-U) changes in several regions of the HIV-1 RNA; commonly, a G-to-A change in the untranslated leader was present exclusively in spliced HIV-1 messenger RNA (mRNA)...

Precision genome editing: a small revolution for glycobiology.

Precise and stable gene editing in mammalian cell lines has until recently been hampered by the lack of efficient targeting methods. While different gene silencing strategies have had tremendous impact on many biological fields, they have generally not been applied with wide success in the field of glycobiology, primarily due to their low efficiencies, with resultant failure to impose substanti...

Efficient Mitochondrial Genome Editing by CRISPR/Cas9

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has been widely used for nuclear DNA editing to generate mutations or correct specific disease alleles. Despite its flexible application, it has not been determined if CRISPR/Cas9, originally identified as a bacterial defense system against virus, can be targeted to mitochondria for mtDNA editing. Here, we show t...

Understanding Cancer Mutations by Genome Editing

Ali, M. A. 2014. Understanding Cancer Mutations by Genome Editing. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1054. 37 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9106-2. Mutational analyses of cancer genomes have identified novel candidate cancer genes with hitherto unknown function in cancer. To enable phenotyping of mutations in suc...