منابع مشابه
Plasma membranes from insect midgut cells.
Plasma membranes from insect midgut cells are separated into apical and basolateral domains. The apical domain is usually modified into microvilli with a molecular structure similar to other animals. Nevertheless, the microvillar structure should differ in some insects to permit the traffic inside them of secretory vesicles that may budd laterally or pinch-off from the tips of microvilli. Other...
متن کاملProteases Detection of invitro Culture of Midgut Cells from Hyalomma anatolicum anatolicum (Acari: Ixodidae)
Proteases play a key role in protein digestion in ticks and other haematophagous insects. Our understanding of blood meal digestion in digestive system of ticks can be very useful for better understanding of basic rules for control of ticks. Cells of the midgut endocytose blood components. Blood proteins uptake by midgut cells, suggesting the presence of proteases in the midgut cells. In this...
متن کاملOptimized primary culture and subculture of granulosa cells
Background: Primordial follicle includes an oocyte surrounded by a layer of somatic cells called granulosa cells (GCs). GCs, also known as nurse cells, are an important protective element for the growth and survival of oocytes. Oocytes, which lack some of the metabolic processes required granulosa cells for their development. Objective: This manuscript has been provided to explain the protocol...
متن کاملPrimary culture of ovarian follicular cells of Sterlet, Acipenser ruthenus to develop an in vitro system
The aim of the present study was to develop an in vitro system for functional investigation of ovarian follicular cells in Sterlet, Acipenser ruthenus. Oocytes for the primary culture were obtained from the ovaries of a 6 years old Sterlet 729 g in weight and 47 cm in total length. The oocytes were in advanced vitellogenesis stage (PI >10). A part of the ovary (containing about 300 follicles) w...
متن کاملproteases detection of invitro culture of midgut cells from hyalomma anatolicum anatolicum (acari: ixodidae)
proteases play a key role in protein digestion in ticks and other haematophagous insects. our understanding of blood meal digestion in digestive system of ticks can be very useful for better understanding of basic rules for control of ticks. cells of the midgut endocytose blood components. blood proteins uptake by midgut cells, suggesting the presence of proteases in the midgut cells. in this s...
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ژورنال
عنوان ژورنال: In Vitro Cellular & Developmental Biology - Animal
سال: 2009
ISSN: 1071-2690,1543-706X
DOI: 10.1007/s11626-009-9211-8